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Lsbad, CA). To assemble His-Ex4a(+){full|complete|total
Lsbad, CA). To assemble His-Ex4a(+)comprehensive expression vector, WT1 cDNALsbad, CA). To build His-Ex4a(+)entire expression vector, WT1 cDNA fragments from exons one to 4a and exons 5 to ten had been PCR-amplified and inserted into EcoRI/NotI and NotI/XbaI web-site, respectively, of pcDNA3.1His vector. 17AA(+)KTS(-)WT1 isoform (WTB) vector and N-terminal His-tagged 17AA(+)KTS (+)WT1 isofor