PR5 Bookmarking Website - Primer set of 4a-F and Ex6-R for Ex Primer set of 4a-F and Ex6-R for Ex4a(+) WT1 amplification and Ex6-F and Ex7-R primer pair for overall WT1 amplification were applied, respectively. The expression amounts of WT1 mRNA were being calculated using the comparative Ct system (2-Ct) with Actin as being the reference gene. Primer sequences for qPCR are as follows: 4a-F, 5'-ATTCCATTGCCTTTC CACAG-3'; Ex6-R, 5'-GACACCGTGCGTGTGTATTC-3'; Ex6 Tue, 13 Mar 2018 20:02:15 UTC en